Date of Award

5-2018

Document Type

Thesis

Degree Name

Bachelor of Science

Department

Biological Sciences

First Advisor

Matthias Falk

Second Advisor

Damien Thevenin

Abstract

Connexin 43 (Cx43) accrual and removal from gap junctions is a complex progress regulated by specific phosphorylations, ubiquitinations and binding and release of regulatory proteins on the C- terminal tail of Cx43. In Thévenin & Margraf et al., 2017, our lab showed that specific phosphorylations on the C-terminal tail of Cx43 occur before and after the binding and release of the scaffold protein Zona Occludens-1 (ZO-1). In Kells-Andrews & Margraf et al., 2018 we show that K63-poly-ubiquitination occurs on residues K264 and K303 of the Cx43 C-terminal tail. This undergraduate thesis summarizes the immunofluorescence imaging and quantification I have contributed to each of these papers, and additional work which further supports the ubiquitination of K264 and K303 on the Cx43 gap junction protein tail.

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